Telomerase-mediated telomere elongation from human blastocysts to embryonic stem cells

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Telomerase-mediated telomere elongation from human blastocysts to embryonic stem cells.

High telomerase activity is a characteristic of human embryonic stem cells (hESCs), however, the regulation and maintenance of correct telomere length in hESCs is unclear. In this study we investigated telomere elongation in hESCs in vitro and found that telomeres lengthened from their derivation in blastocysts through early expansion, but stabilized at later passages. We report that the core u...

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Telomerase - mediated telomere elongation from human blastocysts to embryonic stem cells Running

1 Institute of Reproductive & Stem Cell Engineering, Central South University, Changsha 410001, China 2 National Engineering and Research Center of Human Stem Cell, Changsha 410001, China 3 Reproductive & Genetic Hospital of CITIC-Xiangya, Changsha 410001, China 4 Key laboratory of Stem Cells and Reproductive Engineering, Ministry of Health, Changsha 410008, China * These authors contribute equ...

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Patient-specific embryonic stem cells derived from human SCNT blastocysts.

Patient-specific, immune-matched human embryonic stem cells (hESCs) are anticipated to be of great biomedical importance for studies of disease and development and to advance clinical deliberations regarding stem cell transplantation. Eleven hESC lines were established by somatic cell nuclear transfer (SCNT) of skin cells from patients with disease or injury into donated oocytes. These lines, n...

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Human POT1 Facilitates Telomere Elongation by Telomerase

Mammalian telomeric DNA is mostly composed of double-stranded 5'-TTAGGG-3' repeats and ends with a single-stranded 3' overhang. Telomeric proteins stabilize the telomere by protecting the overhang from degradation or by remodeling the telomere into a T loop structure. Telomerase is a ribonucleoprotein that synthesizes new telomeric DNA. In budding yeast, other proteins, such as Cdc13p, that may...

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Telomerase-mediated telomere elongation provides cell populations with the ability to proliferate indefinitely. Telomerase is capable of recognizing and extending the shortest telomeres in cells; nevertheless, how this mechanism is executed remains unclear. Here, we show that, in the fission yeast Schizosaccharomyces pombe, shortened telomeres are highly transcribed into the evolutionarily cons...

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ژورنال

عنوان ژورنال: Development

سال: 2014

ISSN: 1477-9129,0950-1991

DOI: 10.1242/dev.109116